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anti clip 170  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti clip 170
    Anti Clip 170, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+clip+170/CLIP-170+Antibody/pmc13018197-379-42-45
    Average 93 stars, based on 52 article reviews
    anti clip 170 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: FOXJ1 mediates taxane resistance through regulation of microtubule dynamics
    Article Snippet: Primary antibodies used include anti-GAPDH (Cell Signaling Technology, #2118, 1:3000), anti-Vinculin (Santa Cruz, sc−73614, 1:5,000), anti-Acetyl-α-Tubulin (Cell Signaling Technology, #5335, 1:3000), anti-α-Tubulin (Abcam, ab7291, 1:4000), anti-FOXJ1 (R&D Systems, AF3619, 1:100), anti-TPPP3 (Thermo Fisher, PA5−24925, 1:100), anti-phospho-Histone3 (ser10) (Cell Signaling Technology, #9701, 1:1000), anti-CLIP-170 (clone: E−8, Santa Cruz, 1:500).

    Article Title: FOXJ1 mediates taxane resistance through regulation of microtubule dynamics
    Article Snippet: Primary antibodies used include anti-GAPDH (Cell Signaling Technology, #2118), anti-Vinculin (Santa Cruz, sc-73614), anti-Acetyl-α-Tubulin (Cell Signaling Technology, #5335), anti-α-Tubulin (Abcam, ab7291), anti-FOXJ1 (R&D Systems, AF3619), anti-TPPP3 (Thermo Fisher, PA5-24925), anti-phospho-Histone 3 (ser10) (Cell signaling Technology, #9701), anti-CLIP-170 (clone: E-8, Santa Cruz).

    Article Title: TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells
    Article Snippet: Anti-CLIP-170 [H-300], rabbit polyclonal , Santa Cruz Biotechnology , Cat# sc-25613; RRID:AB_2082238.

    Article Title: FOXJ1 mediates taxane resistance through regulation of microtubule dynamics.
    Article Snippet: Primary antibodies used include anti-GAPDH (Cell Signaling Technology, #2118, 1:3,000), anti-Vinculin (Santa Cruz, sc73614, 1:5,000), anti-Acetyl-α-Tubulin (Cell Signaling Technology, #5335, 1:3,000), anti-αTubulin (Abcam, ab7291, 1:4,000 ), anti-FOXJ1 (R&D Systems, AF3619, 1:100), anti-TPPP3 (Thermo Fisher, PA5-24925, 1:100 ), anti-phospho-Histone3 (ser10) (Cell Signaling Technology, #9701, 1:1,000), anti-CLIP-170 (clone: E-8, Santa Cruz, 1:500).

    Western Blot:

    Article Title: The Ndc80 complex is essential for the initial kinetochore-microtubule capture during early mitosis
    Article Snippet: The primary monoclonal mouse antibodies used were anti-Hec1 9G3 (Abcam, ab3613) for IF at 1:400, and for WB at 1:1000; anti-Dynein Intermediate Chain (DIC) clone 74.1 (EMD Millipore, MAB1618) for IF at 1:300, for WB at 1:1000, anti-CENP-E 1H12 (Abcam, ab5093) IF at 1:400, and anti-α-tubulin DM1A (Santa Cruz, Sc32293) IF at 1:750, WB at 1:1000. .. The primary rabbit polyclonal antibodies used were anti-Hec1 (Bethyl Laboratories, A300-771A), anti-CLIP-170 (Santa Cruz, H-300) for IF at 1:750; anti-Zwint1 (Bethyl, A300-781A) for IF at 1:400; anti-Spindly (a gift from Dr. Reto Gassmann, UC San Diego) for IF at 1:5000, and for WB at 1:1000; anti-CENP-E (Boster, M04553-1) for WB at 1:1000. .. We also used a human anti-centromeric (Immunovision) at 1:500 for IF.

    Article Title: Coordination between the Ndc80 complex and dynein is essential for microtubule plus-end capture by kinetochores during early mitosis.
    Article Snippet: Mitotic kinetochores are initially captured by dynamic microtubules via a “search-and-capture” mechanism.. The microtubule motor, dynein, is critical for kinetochore capture as it has been shown to transport microtubule-attached chromosomes toward the spindle pole during prometaphase.. The microtubule-binding nuclear division cycle 80 (Ndc80) complex that is recruited to kinetochores in prophase is known to play a central role in forming kinetochore-microtubule (kMT) attachments in metaphase.

    Incubation:

    Article Title: A liquid +TIP-network regulates microtubule dynamics through tubulin condensation
    Article Snippet: Cell lysates were boiled run on SDS-PAGE gels (10% acrylamide) and subsequently transferred to a nitrocellulose membrane using an iBLOT 2 Gel Transfer Device (ThermoFisher Scientific, IB21001). .. Nitrocellulose membranes were blocked for 1 h with 5 % dried milk resuspended in TBS-Tween 1 %, then incubated over-night with primary antibodies: anti-beta-tubulin (Sigma, T6074, 1:1000 dilution) anti-EB3 (ATLAS anti-MAPRE3, HPA-009263, 1:500 dilution), or anti-CLIP-170 (Santa Cruz Biotechnology, SC-28325, 1:1000 dilution). .. The following day, unbound antibodies were washed off with TBS-Tween 1%, and membranes were incubated with secondary antibodies conjugated to horseradish peroxidase (anti-mouse or anti-rabbit; GE Healthcare 17097199 and 16951542, 1:5000 dilution) for 1 hour at room temperature.



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